Sodium current in single cells from bullfrog atrium: voltage dependence and ion transfer properties.
نویسندگان
چکیده
منابع مشابه
A time- and voltage-dependent K+ current in single cardiac cells from bullfrog atrium
Individual myocytes were isolated from bullfrog atrium by enzymatic and mechanical dispersion, and a one-microelectrode voltage clamp was used to record the slow outward K+ currents. In normal [K+]o (2.5 mM), the slow outward current tails reverse between -95 and -100 mV. This finding, and the observed 51-mV shift of Erev/10-fold change in [K+]o, strongly suggest that the "delayed rectifier" in...
متن کاملA time-dependent and voltage-sensitive K+ current in single cells from frog atrium
A quantitative description of the time-dependent and voltage-sensitive outward currents in heart has been hampered by the complications inherent to the multicellular preparations previously used. We have used the whole-cell patch-clamp technique to record the delayed outward K+ current, IK, in single cells dissociated from frog atrium. Na+ currents were blocked with tetrodotoxin and Ca2+ curren...
متن کاملATP-activated channels in rat and bullfrog sensory neurons: current-voltage relation and single-channel behavior.
Ionic currents activated by extracellular adenosine 5'-triphosphate (ATP) were studied in voltage-clamped dorsal root ganglion neurons from rats and bullfrogs. Under quasiphysiological ionic conditions, ATP-activated current reversed near 0 mV and showed strong inward rectification. Strong inward rectification was maintained even in symmetric solutions of divalent-free Cs glutamate. Examined wi...
متن کاملActive and passive electrical properties of single bullfrog atrial cells
Single cells from the bullfrog (Rana catesbeiana) atrium have been prepared by using a modification of the enzymatic dispersion procedure described by Bagby et al. (1971. Nature [Long.]. 234:351--352) and Fay and Delise (1973. Proc. Natl. Acad. Sci. U.S.A. 70:641--645). Visualization of relaxed cells via phase-contrast or Nomarski optics (magnification, 400--600) indicates that cells range betw...
متن کاملCurrent-voltage curve of sodium channels and concentration dependence of sodium permeability in frog skin.
1. The inward facing membranes of in vitro frog skin epithelium were depolarized with solutions of high K concentration. The electrical properties of the epithelium are then expected to be governed by the outward facing, Na-selective membrane.2. In this state, the transepithelial voltage (V) was clamped to zero and step-changes of Na activity in the outer solution ((Na)(o)) were performed with ...
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ژورنال
عنوان ژورنال: The Journal of Physiology
سال: 1987
ISSN: 0022-3751
DOI: 10.1113/jphysiol.1987.sp016736